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superscript cdna synthesis kit  (Quanta Biosciences)


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    Quanta Biosciences superscript cdna synthesis kit
    Superscript Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 3869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+synthesis+kit/qScript+cDNA+Synthesis+Kit/bio_rxiv__2025__10__15__682356-329-1-5
    Average 97 stars, based on 3869 article reviews
    superscript cdna synthesis kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the Superscript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence
    Article Snippet: .. Purified RNA was treated with the turbo DNAse kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047–500), per manufacturer instructions. cDNA was diluted 1:150 to further reduce bacterial DNA contamination and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, catalog# 95072-05K) and BioRad CFX96 Real-Time System, C1000 Touch Thermocycler. qRT-PCR primers used in this study can be found in . ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Article Title: NIN-Like Proteins: Interesting Players in Rhizobia-Induced Nitrate Signaling Response During Interaction with Non-Legume Host <i>Arabidopsis thaliana</i>
    Article Snippet: .. RT was accomplished from purified RNA with the SuperScript cDNA Synthesis Kit (Quanta BioScience). ..

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.
    Article Snippet: Briefly, GBS strains were grown to mid- log (OD600 = 0.4– 0.6) and RNA was purified using the MACHEREY- NAGEL NucleoSpin Kit (catalog# 740955.250) according to the manufacturer's instructions with the addition of three bead- beating steps (30 s × 3, with 1- min rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β- mercaptoethanol. .. Purified RNA was treated with the Turbo DNase Kit (Invitrogen, catalog# AM1907) according to the manufacturer's instructions. cDNA was synthesized using the SuperScript cDNA Synthesis Kit (QuantaBio, catalog# 95047- 500), per the manufacturer's instructions. .. All RT- PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio- Rad T100 thermal cycler: 98°C, 2- min hot start; 34 cycles (98°C, 10 s; 60°C, 20 s; 72°C, 30- s extension/kb); and 72°C, 10- min extension.

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection.
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Synthesized:

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the Superscript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence
    Article Snippet: .. Purified RNA was treated with the turbo DNAse kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047–500), per manufacturer instructions. cDNA was diluted 1:150 to further reduce bacterial DNA contamination and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, catalog# 95072-05K) and BioRad CFX96 Real-Time System, C1000 Touch Thermocycler. qRT-PCR primers used in this study can be found in . ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.
    Article Snippet: Briefly, GBS strains were grown to mid- log (OD600 = 0.4– 0.6) and RNA was purified using the MACHEREY- NAGEL NucleoSpin Kit (catalog# 740955.250) according to the manufacturer's instructions with the addition of three bead- beating steps (30 s × 3, with 1- min rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β- mercaptoethanol. .. Purified RNA was treated with the Turbo DNase Kit (Invitrogen, catalog# AM1907) according to the manufacturer's instructions. cDNA was synthesized using the SuperScript cDNA Synthesis Kit (QuantaBio, catalog# 95047- 500), per the manufacturer's instructions. .. All RT- PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio- Rad T100 thermal cycler: 98°C, 2- min hot start; 34 cycles (98°C, 10 s; 60°C, 20 s; 72°C, 30- s extension/kb); and 72°C, 10- min extension.

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection.
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    cDNA Synthesis:

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the Superscript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
    Article Snippet: Moneymaker) was extracted and purified with the ReliaPrep® RNA Tissue Miniprep System (Promega) according to manufacturer’s instructions. .. The Superscript cDNA Synthesis Kit (Quantabio) was used for cDNA synthesis from 1 μg of RNA. qRT-PCR was performed using diluted cDNA (1:8) on a LightCycler 480 (Roche Diagnostics) in 384-well plates with LightCycler 480 SYBR Green I Master (Roche) according to the manufacturer’s instructions. ..

    Article Title: A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence
    Article Snippet: .. Purified RNA was treated with the turbo DNAse kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047–500), per manufacturer instructions. cDNA was diluted 1:150 to further reduce bacterial DNA contamination and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, catalog# 95072-05K) and BioRad CFX96 Real-Time System, C1000 Touch Thermocycler. qRT-PCR primers used in this study can be found in . ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Article Title: NIN-Like Proteins: Interesting Players in Rhizobia-Induced Nitrate Signaling Response During Interaction with Non-Legume Host <i>Arabidopsis thaliana</i>
    Article Snippet: .. RT was accomplished from purified RNA with the SuperScript cDNA Synthesis Kit (Quanta BioScience). ..

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract.
    Article Snippet: Briefly, GBS strains were grown to mid- log (OD600 = 0.4– 0.6) and RNA was purified using the MACHEREY- NAGEL NucleoSpin Kit (catalog# 740955.250) according to the manufacturer's instructions with the addition of three bead- beating steps (30 s × 3, with 1- min rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β- mercaptoethanol. .. Purified RNA was treated with the Turbo DNase Kit (Invitrogen, catalog# AM1907) according to the manufacturer's instructions. cDNA was synthesized using the SuperScript cDNA Synthesis Kit (QuantaBio, catalog# 95047- 500), per the manufacturer's instructions. .. All RT- PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio- Rad T100 thermal cycler: 98°C, 2- min hot start; 34 cycles (98°C, 10 s; 60°C, 20 s; 72°C, 30- s extension/kb); and 72°C, 10- min extension.

    Article Title: Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract
    Article Snippet: Briefly, GBS strains were grown to mid-log (OD 600 = 0.4-0.6) and RNA was purified using the MACHEREY-NAGEL NucleoSpin kit (catalog# 740955.250) according to manufacturer instructions with the addition of three bead beating steps (30 sec x 3, with one minute rest on ice between each) following the resuspension of bacterial pellets in RA1 buffer + β-mercaptoethanol. .. Purified RNA was treated with the Turbo DNase kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047-500), per manufacturer instructions. .. All RT-PCR reactions were performed using Q5 polymerase (New England Biolabs) under the following cycling conditions on a Bio-Rad T100 thermal cycler: 98°C, 2-min hot start; 34 cycles (98°C, 10 seconds; 60 °C, 20 seconds; 72°C, 30-second extension/kb); and 72°C, 10-min extension.

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection.
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Quantitative RT-PCR:

    Article Title: Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
    Article Snippet: Moneymaker) was extracted and purified with the ReliaPrep® RNA Tissue Miniprep System (Promega) according to manufacturer’s instructions. .. The Superscript cDNA Synthesis Kit (Quantabio) was used for cDNA synthesis from 1 μg of RNA. qRT-PCR was performed using diluted cDNA (1:8) on a LightCycler 480 (Roche Diagnostics) in 384-well plates with LightCycler 480 SYBR Green I Master (Roche) according to the manufacturer’s instructions. ..

    Article Title: A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence
    Article Snippet: .. Purified RNA was treated with the turbo DNAse kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047–500), per manufacturer instructions. cDNA was diluted 1:150 to further reduce bacterial DNA contamination and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, catalog# 95072-05K) and BioRad CFX96 Real-Time System, C1000 Touch Thermocycler. qRT-PCR primers used in this study can be found in . ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection.
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    SYBR Green Assay:

    Article Title: Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
    Article Snippet: Moneymaker) was extracted and purified with the ReliaPrep® RNA Tissue Miniprep System (Promega) according to manufacturer’s instructions. .. The Superscript cDNA Synthesis Kit (Quantabio) was used for cDNA synthesis from 1 μg of RNA. qRT-PCR was performed using diluted cDNA (1:8) on a LightCycler 480 (Roche Diagnostics) in 384-well plates with LightCycler 480 SYBR Green I Master (Roche) according to the manufacturer’s instructions. ..

    Article Title: A type VII secretion system in Group B Streptococcus mediates cytotoxicity and virulence
    Article Snippet: .. Purified RNA was treated with the turbo DNAse kit (Invitrogen, catalog# AM1907) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, catalog# 95047–500), per manufacturer instructions. cDNA was diluted 1:150 to further reduce bacterial DNA contamination and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, catalog# 95072-05K) and BioRad CFX96 Real-Time System, C1000 Touch Thermocycler. qRT-PCR primers used in this study can be found in . ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..

    Article Title: Enhanced Vulnerability of Diabetic Mice to Hypervirulent Streptococcus agalactiae ST-17 Infection.
    Article Snippet: .. RNA was purified using the Machery-Nagel Nucleospin kit and treated with the turbo DNAse kit (Invitrogen, San Diego, CA, USA) according to manufacturer instructions. cDNA was synthesized using the SuperScript cDNA synthesis kit (QuantaBio, Beverly, MA, USA) per manufacturer instructions. cDNA was diluted 1:250 to further reduce bacterial DNA contamination, and qRT-PCR was performed using PerfeCTa SYBR Green (QuantaBio, Beverly, MA, USA), BioRad CFX96 Real-Time System, and C1000 Touch Thermocycler. ..



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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control